10310 Enpp7 Status: Cancelled
| Gene | Enpp7 |
| Chromosome | 11 |
| Deletion Start | 118,988,202 |
| Deletion End | 118,992,845 |
| Deletion Size | 4,644 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,989,027 | 118,989,045 | GGCAGCCATCACAGCTCAG |
| TUP | 118,989,049 | 118,989,071 | TGGTCTGAGGCCTGAACGTCCTG |
| TUR | 118,989,090 | 118,989,110 | AGCCTTGGTCTCCCTTTCTTC |
| TDF | 118,992,735 | 118,992,758 | TCCAGATTCTCACTCTTTCCCTTC |
| TDP | 118,992,764 | 118,992,787 | TTCACCCACTCCCACTTCCAGTGC |
| TDR | 118,992,796 | 118,992,814 | TCCAGATGCTGCTGTGTTC |
PCR Primers
| Primer | Sequence |
| SU | GGAAGGACCCTGAAGGATAG |
| SD | GCACTCCAGAACCAGCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 318 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 310 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
