10315 Map1lc3a Status: Cancelled
| Gene | Map1lc3a |
| Chromosome | 2 |
| Deletion Start | 155,276,593 |
| Deletion End | 155,277,662 |
| Deletion Size | 1,070 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,276,956 | 155,276,974 | AGCCGACCGCTGTAAGGAG |
| TUP | 155,276,978 | 155,276,999 | CAGCAGATCCGCGACCAGCACC |
| TUR | 155,277,000 | 155,277,020 | GACTCACCGGGATCTTACTGG |
| TDF | 155,277,503 | 155,277,523 | GCCTTCTTCCTGCTGGTCAAC |
| TDP | 155,277,527 | 155,277,550 | CACAGCATGGTGAGCGTCTCCACA |
| TDR | 155,277,572 | 155,277,595 | GAATCCGTCTTCATCCTTCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | TTCCGTCGGCCCAATGGGA |
| SD | GATGGGCAGACAGGCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 349 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
