10316 Dusp18 Status: Cancelled
| Gene | Dusp18 |
| Chromosome | 11 |
| Deletion Start | 3,897,015 |
| Deletion End | 3,897,823 |
| Deletion Size | 587 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,897,136 | 3,897,158 | CCAGCAATCAGATCACCACAGTC |
| TUP | 3,897,160 | 3,897,188 | TCAACGTCTCAGTAGAGGTAGCAAACACC |
| TUR | 3,897,206 | 3,897,224 | CACAGGCACCTGCACATAC |
| TDF | 3,897,430 | 3,897,447 | TCATCCGGCCCAACAGTG |
| TDP | 3,897,451 | 3,897,477 | TTTGGGAGCAGCTCATCCATTACGAGT |
| TDR | 3,897,506 | 3,897,525 | CCATTGGCGAGTCCATCATC |
PCR Primers
| Primer | Sequence |
| SU | CGTCCCTTCCATTGTTCAC |
| SD | TCTGAAAGCACAGATGACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 390 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 132 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
