10317 Alyref Status: Cancelled
| Gene | Alyref |
| Chromosome | 11 |
| Deletion Start | 120,598,335 |
| Deletion End | 120,594,834 |
| Deletion Size | 3,502 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,597,643 | 120,597,664 | GACTTCGGAGTGTCAGATGCTG |
| TUP | 120,597,615 | 120,597,640 | ATTCAGGTAGGAGACGGGAGCTCTCC |
| TUR | 120,597,554 | 120,597,577 | GAGGGCAGGTTCCAATCTGTAAAG |
| TDF | 120,595,329 | 120,595,350 | TGGAGTGAGACTTCTTGTGTTC |
| TDP | 120,595,298 | 120,595,321 | TAGGCCGCCCTATGAACATCCAGC |
| TDR | 120,595,260 | 120,595,280 | TGCAGGTCTTCGCTGTGTATC |
PCR Primers
| Primer | Sequence |
| SU | GTTGCCTCCGCGCGGGAATG |
| SD | ACTCAGTACAAAACAGGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 347 bp | - | + | + |
| TUF | TUR | 111 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 339 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
