10318 Slfn2 Status: Cancelled
| Gene | Slfn2 |
| Chromosome | 11 |
| Deletion Start | 83,065,294 |
| Deletion End | 83,070,330 |
| Deletion Size | 5,058 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,065,390 | 83,065,413 | TCATTTCTGTCCCTCACTTCCAAC |
| TUP | 83,065,414 | 83,065,440 | CCTGGGTACAGTCTAGTTGCTCTGGGA |
| TUR | 83,065,458 | 83,065,478 | GGAGCCAAGATGCCAGTAGAG |
| TDF | 83,069,738 | 83,069,758 | AAACTGGAGGGAGACCCTGTG |
| TDP | 83,069,759 | 83,069,782 | TCAGACCAGAGTTGCCTGCAAGCA |
| TDR | 83,069,821 | 83,069,844 | GTTAAACAATGCAGCAGCCAAATC |
PCR Primers
| Primer | Sequence |
| SU | TTCCTGGAGGGTGACAGTTC |
| SD | GGTGGACAAAGGGTAGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 286 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
