10319 Svs3a Status: Cancelled
| Gene | Svs3a |
| Chromosome | 2 |
| Deletion Start | 164,289,292 |
| Deletion End | 164,290,356 |
| Deletion Size | 1,065 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,289,598 | 164,289,621 | GGACACTTCCTAGTAAAAACCTCC |
| TUP | 164,289,634 | 164,289,662 | ATTGGGAAAAACCAGTTCCTCTATGGGCA |
| TUR | 164,289,722 | 164,289,744 | AGCATCAGCATCCTGGCCATATT |
| TDF | 164,289,916 | 164,289,937 | CAGCTAAAGACCATAGGGCAGG |
| TDP | 164,255,929 | 164,255,955 | CTGAAATCCCACGGAGCCCCTCTGAAG |
| TDR | 164,289,986 | 164,290,006 | GCAGATTAAGGCGTGCTTTGA |
PCR Primers
| Primer | Sequence |
| SU | AGAGCCCACACTTGAACTGC |
| SD | GCAATGTAGCAAGAAACTGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
