10320 4930579C15Rik Status: ES cell colonies screened / QC no positives
| Gene | 4930579C15Rik |
| Chromosome | 4 |
| Deletion Start | 94,928,749 |
| Deletion End | 94,907,173 |
| Deletion Size | 21,577 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,927,837 | 94,927,857 | GGCTGTCTTTGCCACTGTTTC |
| TUP | 94,927,803 | 94,927,830 | CCATGGTTCATCATGGCATTGTCACTCC |
| TUR | 94,927,770 | 94,927,793 | GCCCTCCAAATTAGATCAGTTGTC |
| TDF | 94,907,608 | 94,907,628 | GCAGGCTCCAATCAAACAGTC |
| TDP | 94,907,581 | 94,907,605 | AACCGACGAGTCAAGCTTCCTCCCT |
| TDR | 94,907,537 | 94,907,555 | TCCTTACCCGGCTCCTCAG |
PCR Primers
| Primer | Sequence |
| SU | CAAAAGTGAAATGACCATGAG |
| SD | CTTCCTGACCAGTGAAAGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 289 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 347 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
