10321 Gstm1 Status: Cancelled
| Gene | Gstm1 |
| Chromosome | 3 |
| Deletion Start | 108,017,772 |
| Deletion End | 108,012,667 |
| Deletion Size | 5,106 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,017,690 | 108,017,711 | GACTGGGATGTGAAGCAGAAAG |
| TUP | 108,017,661 | 108,017,686 | TTAAGAGCAGGAGCCCTTCCTACAGC |
| TUR | 108,017,616 | 108,017,634 | TGAGAGCTACAGGCAGGAG |
| TDF | 108,012,855 | 108,012,875 | AGGACAAGTGAGCCAGGAGAC |
| TDP | 108,012,835 | 108,012,854 | CCGTGCGTGCATGCGTGCAT |
| TDR | 108,012,785 | 108,012,806 | ATTGGGAAGGACACAGCAGGAG |
PCR Primers
| Primer | Sequence |
| SU | GCCGCTAAGGATCTTAACC |
| SD | CAGGGAGCCAATGAAGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 311 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
