| Gene | Exosc3 |
| Chromosome | 4 |
| Deletion Start | 45,320,588 |
| Deletion End | 45,316,797 |
| Deletion Size | 3,792 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,319,943 | 45,319,967 | CAAATGGAGTGGGTTTCCTTGAATG |
| TUP | 45,319,907 | 45,319,933 | TTGTGCCACCAGACTTGTAAAGGATGC |
| TUR | 45,319,847 | 45,319,869 | CGCACCAGTCCAACTTTATGAAC |
| TDF | 45,317,416 | 45,317,435 | TGACCAGCCTACCGTGAAAG |
| TDP | 45,317,389 | 45,317,411 | ACTGAGCTGGGCACTGTGTCCCA |
| TDR | 45,317,354 | 45,317,374 | GTAGGTGTGAGAACGCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | GTGGGAGATTGTTACATTAG |
| SD | ATTAATGTGTTCAACATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 308 bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 400 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10322B-D8 | VGB6 | C57BL/6NTac | |
| 10322B-D12 | VGB6 | C57BL/6NTac | |
| 10322B-E11 | VGB6 | C57BL/6NTac | |
| 10322B-F12 | VGB6 | C57BL/6NTac | |
