10322 Exosc3 Status: ES cell clone microinjected
| Gene | Exosc3 |
| Chromosome | 4 |
| Deletion Start | 45,333,460 |
| Deletion End | 45,329,669 |
| Deletion Size | 3,792 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,332,815 | 45,332,839 | CAAATGGAGTGGGTTTCCTTGAATG |
| TUP | 45,332,779 | 45,332,805 | TTGTGCCACCAGACTTGTAAAGGATGC |
| TUR | 45,332,719 | 45,332,741 | CGCACCAGTCCAACTTTATGAAC |
| TDF | 45,330,288 | 45,330,307 | TGACCAGCCTACCGTGAAAG |
| TDP | 45,330,261 | 45,330,283 | ACTGAGCTGGGCACTGTGTCCCA |
| TDR | 45,330,226 | 45,330,246 | GTAGGTGTGAGAACGCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | GTGGGAGATTGTTACATTAG |
| SD | ATTAATGTGTTCAACATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 308 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | 400 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
