10327 Fam46b Status: Cancelled
| Gene | Fam46b |
| Chromosome | 4 |
| Deletion Start | 133,480,285 |
| Deletion End | 133,487,103 |
| Deletion Size | 6,819 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,480,636 | 133,480,660 | CGGGAAGCTAGGGAAACTAAGAAAC |
| TUP | 133,480,666 | 133,480,693 | CCCGACTACTAGTTCAGCTCTTCCTGCA |
| TUR | 133,480,699 | 133,480,717 | GGCAAGCAGGCAAGCTCAG |
| TDF | 133,486,404 | 133,486,423 | GAGCGGTAAGAACGTGGAAC |
| TDP | 133,486,424 | 133,486,449 | TCAAGTTCGTGGACTCTGTGAGACGC |
| TDR | 133,486,489 | 133,486,508 | CCAAAGAGGAGCAGCGAGTC |
PCR Primers
| Primer | Sequence |
| SU | TGTGACAGCTGTGCTGACC |
| SD | TACAGTATGATGACCAATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 354 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 298 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
