| Gene | Gpr139 |
| Chromosome | 7 |
| Deletion Start | 119,184,371 |
| Deletion End | 119,184,261 |
| Deletion Size | 111 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,184,340 | 119,184,358 | CCCACCTCGCTGCGAATAG |
| TUP | 119,184,327 | 119,184,339 | CTCGGCTTGCGGC |
| TUR | 119,184,303 | 119,184,321 | GTAGACCACCGGCACGAAG |
| TDF | 119,184,332 | 119,184,350 | GCTGCGAATAGCTCGGCTT |
| TDP | 119,184,310 | 119,184,330 | CGGCTTAGGCTTCGTGCCGGT |
| TDR | 119,184,283 | 119,184,303 | AGGCACAGCAAGAAGCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTCTGAGGCGGGAGCTGAGC |
| SD | ACACCCACAAGTCACAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 56 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10338B-A5 | VGB6 | C57BL/6NTac | |
| 10338B-D2 | VGB6 | C57BL/6NTac | |
| 10338B-D12 | VGB6 | C57BL/6NTac | |
| 10338B-G11 | VGB6 | C57BL/6NTac | |
| 10338B-H4 | VGB6 | C57BL/6NTac | |
