10342 Lcn4 Status: Cancelled
| Gene | Lcn4 |
| Chromosome | 2 |
| Deletion Start | 26,671,264 |
| Deletion End | 26,667,856 |
| Deletion Size | 3,409 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,670,747 | 26,670,767 | GAGAAAGTGTGGGCTTAGGAC |
| TUP | 26,670,721 | 26,670,746 | TCTGTCTACTCCAGCTCTCAGGTGTC |
| TUR | 26,670,692 | 26,670,715 | CTTTGACTCACTGTGGCCTTTATG |
| TDF | 26,668,207 | 26,668,225 | GCTTAGCCAGGAAGCAGTG |
| TDP | 26,668,175 | 26,668,201 | TCCAGGAGGAGTCCTCAAGTGGAATTC |
| TDR | 26,668,124 | 26,668,143 | TGCAAAGGCAGAACTCAAGG |
PCR Primers
| Primer | Sequence |
| SU | AGATGGCGATGACTTTGAAG |
| SD | CTGCTAATCCTAATATCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 327 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 354 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
