| Gene | Ssxb9 |
| Chromosome | X |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,369,785 | 8,369,807 | GGTCCTCCATAAGTCTCAGGATG |
| TUP | 8,369,812 | 8,369,837 | TCCATGCACAAATGGTCCATGAGTCC |
| TUR | 8,369,873 | 8,369,900 | GAGTGTCACCAAATGAATGTAGTACTAC |
| TDF | 8,373,902 | 8,373,922 | GTGCCTAATTCCTCGCTTGAG |
| TDP | 8,373,876 | 8,373,900 | CACACTGACACCCTTCCTCCCTACC |
| TDR | 8,373,850 | 8,373,875 | CAAGAGGCAATAAAAGGGAATCTAGA |
PCR Primers
| Primer | Sequence |
| SU | AGAATCTAATTGATTCCTAACC |
| SD | AGAAATGGCCTACACTTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
