10345 Mageh1 Status: ES cell colonies screened / QC no positives
| Gene | Mageh1 |
| Chromosome | X |
| Deletion Start | 153,037,335 |
| Deletion End | 153,036,649 |
| Deletion Size | 687 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,037,221 | 153,037,239 | CCGATGGCGGCTTCTGTAG |
| TUP | 153,037,194 | 153,037,218 | CCGAGCACACCCGAAGAATACCTGA |
| TUR | 153,037,163 | 153,037,182 | TCCAGGGTGCTTGTGTCTTC |
| TDF | 153,036,856 | 153,036,878 | GTCCAGTGGAGTATGAGTTCTTC |
| TDP | 153,036,825 | 153,036,849 | CCTCGAGCACACGTGGAATCTAGCA |
| TDR | 153,036,770 | 153,036,790 | CAGTCTTTGGAGCATCGGTTC |
PCR Primers
| Primer | Sequence |
| SU | TGCGCAAGTCAGCTTGCCTG |
| SD | TGTAATCTTCTTCCACTCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 295 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 482 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
