10346 Rraga Status: ES cell colonies screened / QC no positives
| Gene | Rraga |
| Chromosome | 4 |
| Deletion Start | 86,575,906 |
| Deletion End | 86,576,943 |
| Deletion Size | 1,038 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,576,342 | 86,576,362 | AAAGAGCGAGAGGAAGACCTG |
| TUP | 86,576,366 | 86,576,387 | CGTTTGTCTCGCCCGCTGGAGT |
| TUR | 86,576,404 | 86,576,423 | GCGTCTCATCCCAGATGGAC |
| TDF | 86,576,561 | 86,576,581 | TCCCACTACCAGTGCAAAGAG |
| TDP | 86,576,582 | 86,576,604 | CAGCGAGATGTGCACCGCTTTGA |
| TDR | 86,576,620 | 86,576,643 | CAGCTCAGCTTAAACTGCTTGATG |
PCR Primers
| Primer | Sequence |
| SU | CGGCTGTGGCCATTGCCTC |
| SD | GTAACATTCCACCTTTGTGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 303 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 341 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
