10347 Mpp2 Status: Cancelled
| Gene | Mpp2 |
| Chromosome | 11 |
| Deletion Start | 102,085,327 |
| Deletion End | 102,059,294 |
| Deletion Size | 26,034 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,085,093 | 102,085,116 | TGTTCCAATAGTGCTTGCTGTATG |
| TUP | 102,085,072 | 102,085,092 | TTGCAGCATGGCTCACCTGCC |
| TUR | 102,085,017 | 102,085,035 | CATTCCCGTCCAGCTTCAC |
| TDF | 102,060,076 | 102,060,094 | AGGTTGGGCTTGCATCTTC |
| TDP | 102,060,053 | 102,060,075 | TGATGACCTGGTCCTGGCCTTGG |
| TDR | 102,060,018 | 102,060,036 | CGAGGCCCTAGCTGCATTG |
PCR Primers
| Primer | Sequence |
| SU | ATGCCATCTGCCTTTCTTGC |
| SD | CCTTAGAAAAGGGTGTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 324 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
