10350 Csf2ra Status: Cancelled
| Gene | Csf2ra |
| Chromosome | 19 |
| Deletion Start | 61,227,362 |
| Deletion End | 61,224,971 |
| Deletion Size | 2,392 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,226,477 | 61,226,495 | TGCATCGTGGGTGTGGTTG |
| TUP | 61,226,451 | 61,226,474 | CAATCACGTCATCACGTCGCCGGT |
| TUR | 61,226,428 | 61,226,447 | ATAGCGGGTTCAGGGCTCAC |
| TDF | 61,225,805 | 61,225,824 | CAGAGCGCAGAGCCAGGAAG |
| TDP | 61,225,783 | 61,225,804 | CACACCCCGGAAGGTGACCTGA |
| TDR | 61,225,732 | 61,225,751 | TGCCTCAGGTCCTCTCCATC |
PCR Primers
| Primer | Sequence |
| SU | GGATAACGTCACCAAGAGAG |
| SD | TCACGGTGACATCAATGTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 298 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 293 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
