| Gene | Adm2 |
| Chromosome | 15 |
| Deletion Start | 89,323,291 |
| Deletion End | 89,324,386 |
| Deletion Size | 1,114 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,323,321 | 89,323,341 | GGTTGCATCAGCCTCCTCTAC |
| TUP | 89,323,348 | 89,323,366 | CCCGGCACGTTGTCTGGCA |
| TUR | 89,323,403 | 89,323,423 | GTTCGGTCCAACCCTGTACTC |
| TDF | 89,324,092 | 89,324,113 | TGCTATGGTTCATCTGCCTCAG |
| TDP | 89,324,119 | 89,324,139 | TGGCTCACGACACCCTGGTCC |
| TDR | 89,324,218 | 89,324,238 | CACAGGCGATGGCTAAGATTC |
PCR Primers
| Primer | Sequence |
| SU | GACAACAGACGCAGGTACC |
| SD | CTTCTTCCAAGCACCTGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 303 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 147 bp | + | + | - |
| NeoFwd | SD | 235 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10353A-A9 | VGB6 | C57BL/6NTac | |
| 10353A-B4 | VGB6 | C57BL/6NTac | |
| 10353A-C12 | VGB6 | C57BL/6NTac | |
| 10353A-G1 | VGB6 | C57BL/6NTac | |
