10355   Cacng5     Status: ES cell colonies screened / QC no positives

GeneCacng5
Chromosome11
Deletion Start107,884,538
Deletion End107,877,314
Deletion Size7,225
Genome Build38
MGI IDMGI:2157946
NCBI Gene ID140723
BAC ID420O13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF107,884,437107,884,456GCACGGACTACTGGCTCTAC
TUP107,884,409107,884,435TGGAAGAAGGCATAATCCTCCCACAGA
TUR107,884,374107,884,394CGAGTGCAGGGACATCTTGAC
TDF107,877,418107,877,436CGACGCCTCGCTACAGATG
TDP107,877,386107,877,412CAACTACCCAGCTTTGCTCAAGTGCCC
TDR107,877,348107,877,365TGGCTCAGCAGGGAGATG

PCR Primers

PrimerSequence
SUGGGAAACTCAGAAGACAAGG
SDGCTATGATTGGCACTCTAGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev364 bp-++
TUFTUR83 bp++-
TDFTDR89 bp++-
NeoFwdSD295 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image