10357 Ell3 Status: Cancelled
| Gene | Ell3 |
| Chromosome | 2 |
| Deletion Start | 121,442,544 |
| Deletion End | 121,439,423 |
| Deletion Size | 3,122 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,441,728 | 121,441,746 | TGGTGGCTTGGACCTTGTG |
| TUP | 121,441,702 | 121,441,726 | ACCAACGCTTAGGCAGGTAAGGACA |
| TUR | 121,441,672 | 121,441,693 | CTTCCCATTTCCTGCAAACTTC |
| TDF | 121,439,999 | 121,440,021 | AGGCAATACAGAGCCATCCACAG |
| TDP | 121,439,972 | 121,439,997 | ACAGAGCAGCAACAGGCCTATGAGCA |
| TDR | 121,439,946 | 121,439,968 | ACTCAGCATAGTCGGTCTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTGAGGTGAGTGAGGTAC |
| SD | TGGCACAGCTGAGGAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 273 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 265 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
