10361 Krtap1-5 Status: ES cell colonies screened / QC no positives
| Gene | Krtap1-5 |
| Chromosome | 11 |
| Deletion Start | 99,580,962 |
| Deletion End | 99,580,548 |
| Deletion Size | 415 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,598,008 | 99,598,026 | GCTTTCCCACTTGCTCCAC |
| TUP | 99,591,249 | 99,591,269 | TGGCACCTGTGGCTCCAGCTG |
| TUR | 99,580,838 | 99,580,856 | CCTGTCCCACAGCAGCTTG |
| TDF | 99,597,715 | 99,597,733 | TGCCGTCCATCCTACTGTG |
| TDP | 99,590,864 | 99,590,882 | ACAGTCCTGCTGCCGCCCA |
| TDR | 99,580,599 | 99,580,618 | AGCAGCAGTAGCAGCAACAG |
PCR Primers
| Primer | Sequence |
| SU | GAACATGGAAACCACAAACGC |
| SD | TGTGGGTTCTCAGCATTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 398 bp | - | + | + |
| TUF | TUR | 17189 bp | + | + | - |
| TDF | TDR | 17135 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
