10363 Dmrta1 Status: ES cell colonies screened / QC one positive
| Gene | Dmrta1 |
| Chromosome | 4 |
| Deletion Start | 89,688,292 |
| Deletion End | 89,692,277 |
| Deletion Size | 3,986 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,689,129 | 89,689,150 | CATCCCGCGTCTAAAGACAATG |
| TUP | 89,689,167 | 89,689,186 | TGGGATCACAGCCGCCGGCT |
| TUR | 89,689,192 | 89,689,211 | TGCGCCTAAATCCACTCCAG |
| TDF | 89,691,709 | 89,691,727 | CCAGAGCCAGCCTTTCCAC |
| TDP | 89,691,730 | 89,691,753 | TGACCTCAAGACCACGAGACCCTC |
| TDR | 89,691,781 | 89,691,802 | TCTAGGCGGCTATGCTTGTAAC |
PCR Primers
| Primer | Sequence |
| SU | AGCCTGGCCCTTCTAGGCTC |
| SD | CACTATAAACTGAACTCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 436 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
