| Gene | Dcxr |
| Chromosome | 11 |
| Deletion Start | 120,727,226 |
| Deletion End | 120,725,484 |
| Deletion Size | 1,743 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,726,466 | 120,726,488 | CGTGGACCTTCTGGTGAACAATG |
| TUP | 120,726,444 | 120,726,464 | TGCTGTGGCTCTGTTGCAGCC |
| TUR | 120,726,400 | 120,726,419 | TGCTGGCTTACGTGTCACAG |
| TDF | 120,726,007 | 120,726,026 | CACGACAACCCTCCTCACAG |
| TDP | 120,725,983 | 120,726,005 | TTCCACCAAGGGTGCTCTGGACA |
| TDR | 120,725,953 | 120,725,972 | CAAGCTCTAGGGCCATCATC |
PCR Primers
| Primer | Sequence |
| SU | ATTCTGCCCTCTATAGTTGG |
| SD | TTCTGGAAGATCCGACTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 355 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 286 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10365B-B10 | VGB6 | C57BL/6NTac | |
| 10365B-D6 | VGB6 | C57BL/6NTac | |
| 10365B-D9 | VGB6 | C57BL/6NTac | |
| 10365B-D11 | VGB6 | C57BL/6NTac | |
| 10365B-E12 | VGB6 | C57BL/6NTac | |
| 10365B-G1 | VGB6 | C57BL/6NTac | |
