10370 Kctd11 Status: ES cell colonies screened / QC no positives
| Gene | Kctd11 |
| Chromosome | 11 |
| Deletion Start | 69,880,207 |
| Deletion End | 69,879,417 |
| Deletion Size | 791 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,880,050 | 69,880,067 | CCGTGGGTACGGAGAAAC |
| TUP | 69,880,026 | 69,880,049 | TGCCCTTCTTAAGGCAGAGGCTGA |
| TUR | 69,880,007 | 69,880,025 | GGGCCGGATCTGGTAGAAG |
| TDF | 69,879,540 | 69,879,561 | ACCCTGAAGACCTTCTGAACTC |
| TDP | 69,879,515 | 69,879,536 | ATCCTTGCGCTTTGTGCGCCAC |
| TDR | 69,879,490 | 69,879,509 | AGTCAAGTGGAGGGCAGCAC |
PCR Primers
| Primer | Sequence |
| SU | GTCTTGTTCTCCAACCTCTG |
| SD | TCTTACTTTGCCACACTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 61 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
