10371 1700018C11Rik Status: Cancelled
| Gene | 1700018C11Rik |
| Chromosome | 4 |
| Deletion Start | 63,612,444 |
| Deletion End | 63,607,290 |
| Deletion Size | 5,155 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,611,951 | 63,611,971 | CACCAAACCCTTGCCTCAAAG |
| TUP | 63,611,920 | 63,611,947 | TTCAGTTTATGCTGCTGGGACTGTGAGG |
| TUR | 63,611,894 | 63,611,916 | AACCTTGGCGCTCTCAATAACAG |
| TDF | 63,607,663 | 63,607,681 | GCAGCCTACCAGGGAACAC |
| TDP | 63,607,633 | 63,607,654 | TCTAAGCCAGGACGGGCCCAAG |
| TDR | 63,607,576 | 63,607,599 | GGTGGAGTCACTATCAACAGAATG |
PCR Primers
| Primer | Sequence |
| SU | AGCCTGATTACCAGTGATCTAC |
| SD | ACTGTAAGATCTGAGGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 344 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
