10374 Gpr137 Status: Cancelled
| Gene | Gpr137 |
| Chromosome | 19 |
| Deletion Start | 6,940,836 |
| Deletion End | 6,938,314 |
| Deletion Size | 2,542 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,940,497 | 6,940,517 | TCACACTGACGCTTATGAACC |
| TUP | 6,940,471 | 6,940,496 | TCTACTTTGTGCAGGTAACCACGGGA |
| TUR | 6,940,434 | 6,940,455 | ACCATTGAGATTGCCCTCCTAC |
| TDF | 6,938,580 | 6,938,600 | CTCAGCCTCTCCCATCTCAAG |
| TDP | 6,938,558 | 6,938,578 | ATGTGGCCCTGGGCACTGCCT |
| TDR | 6,938,531 | 6,938,548 | TGCGGGCAGAGAAACAAG |
PCR Primers
| Primer | Sequence |
| SU | CCTGGGTAGGAAAGACCAC |
| SD | TTGAGTGGTAGGCACAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 374 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
