10375 Fbxo48 Status: Cancelled
| Gene | Fbxo48 |
| Chromosome | 11 |
| Deletion Start | 16,953,354 |
| Deletion End | 16,954,341 |
| Deletion Size | 988 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,953,482 | 16,953,503 | AACTACTGCCCTTGGAAGTCAC |
| TUP | 16,953,515 | 16,953,544 | TCAGCCAGCTAGACATTCAGAGTTTATGCA |
| TUR | 16,953,554 | 16,953,571 | TCCAACCTGTGCATGTCC |
| TDF | 16,954,049 | 16,954,073 | GTGTGTAATTCATCTCAGTCCTGTG |
| TDP | 16,954,076 | 16,954,101 | TGAGTTGCTCTGCTTGCAGACATGCG |
| TDR | 16,954,102 | 16,954,120 | GCAGCATGGTTGGCTGGTG |
PCR Primers
| Primer | Sequence |
| SU | GGGAATGTGTTCATGGTCTC |
| SD | CATTTCAAAATCATTCTACCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 374 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 301 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
