10382 Ccin Status: Cancelled
| Gene | Ccin |
| Chromosome | 4 |
| Deletion Start | 43,983,598 |
| Deletion End | 43,985,366 |
| Deletion Size | 1,788 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,984,052 | 43,984,071 | ACTCTGGCATTCGTGACAAC |
| TUP | 43,984,072 | 43,984,093 | TTCCACTTCTGGGCCAGTCCCG |
| TUR | 43,984,128 | 43,984,146 | GAGCAGGCGACCGAAGATG |
| TDF | 43,985,070 | 43,985,091 | ACAGAAGATCAAGGCCAACAAG |
| TDP | 43,985,092 | 43,985,117 | TCGACAACCTCAGTACCTCTGTTGCC |
| TDR | 43,985,123 | 43,985,143 | GGGATACATCCAAGGGACAAC |
PCR Primers
| Primer | Sequence |
| SU | AAACAACCATCAGTACAAAC |
| SD | GTGTAGCCCCAAGCATTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 226 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 197 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
