10384 Krtap31-1 Status: Cancelled
| Gene | Krtap31-1 |
| Chromosome | 11 |
| Deletion Start | 99,907,958 |
| Deletion End | 99,936,880 |
| Deletion Size | 28,923 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,907,991 | 99,908,009 | CCATGCTGCTGCAAGACCG |
| TUP | 99,936,384 | 99,936,403 | CCTGCAGGACCTCCAGCAGC |
| TUR | 99,936,414 | 99,936,431 | GGCAGCTGGATTCAGAGC |
| TDF | 99,908,360 | 99,908,380 | CCCTCCTGTGTGATCATTGGT |
| TDP | 99,936,757 | 99,936,777 | CACACCCTGCTGCCAGCCCTG |
| TDR | 99,908,432 | 99,908,453 | TTTGTTCCTTTGGGAGCTGGTT |
PCR Primers
| Primer | Sequence |
| SU | CTTCAAGTACGTTATCACCAG |
| SD | TTCTTAGGGAGGAAGTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 357 bp | - | + | + |
| TUF | TUR | 28441 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 28258 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
