10388 E130306D19Rik Status: Cancelled
| Gene | E130306D19Rik |
| Chromosome | 4 |
| Deletion Start | 43,499,604 |
| Deletion End | 43,496,658 |
| Deletion Size | 2,947 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,499,301 | 43,499,324 | TCCTGAAAGCCAAAGGAACACTAC |
| TUP | 43,499,273 | 43,499,299 | ACCACCAGAACTCCAGACCCTGTTTGG |
| TUR | 43,499,215 | 43,499,235 | TCCCTCTCCCTGCAATTCTTC |
| TDF | 43,497,645 | 43,497,665 | TCGGCCAGAGTCAGAAGAATG |
| TDP | 43,497,620 | 43,497,641 | AGCATCTGCTGCGTGTCCAGGC |
| TDR | 43,497,569 | 43,497,589 | GTCCACGGTCTACCTGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | ACATTCCCAATTCTCACTTAG |
| SD | AATGGACTCCTTTTCAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 427 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 346 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
