10393 Gm561 Status: ES cell colonies screened / QC no positives
| Gene | Gm561 |
| Chromosome | 2 |
| Deletion Start | 144,594,094 |
| Deletion End | 144,595,256 |
| Deletion Size | 1,163 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 144,594,458 | 144,594,477 | GTGTCGTGCTAGGTGGATTC |
| TUP | 144,594,483 | 144,594,505 | CCTCGGACAGTAGTCCGGGTGCT |
| TUR | 144,594,507 | 144,594,531 | CATGCGACAGTTTCAAGGAAATGAG |
| TDF | 144,594,696 | 144,594,718 | AGGTGACTCTGAGTGGCTGAAAG |
| TDP | 144,594,719 | 144,594,742 | TCCCTCTAAAATCGGTTGCGGTGC |
| TDR | 144,594,745 | 144,594,765 | AGTCTATTCGGCTTGGGCAAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTCCCCAGCATATAGTAC |
| SD | TACACACATGCATCCGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
