10394 Dem1 Status: Germline transmission achieved
| Gene | Dem1 |
| Chromosome | 4 |
| Deletion Start | 120,595,268 |
| Deletion End | 120,594,128 |
| Deletion Size | 1,141 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,595,162 | 120,595,185 | GGATCTGGAAGAAGCCAAAGAATC |
| TUP | 120,595,134 | 120,595,158 | TGTTTCACTTAGCAAGCCTGGCCCT |
| TUR | 120,595,107 | 120,595,127 | GTCATCCTTCCCAGGGAGTTC |
| TDF | 120,594,297 | 120,594,315 | GCAGCATTACGTGGCCTAC |
| TDP | 120,594,270 | 120,594,293 | ATGGGCCACCGAGATCCTCAAGGC
|
| TDR | 120,594,211 | 120,594,233 | CACTCGCAGATGTCCACATAGTC |
PCR Primers
| Primer | Sequence |
| SU | GTGTTCTCCCTCTAGAAGTG |
| SD | AAGAATTTTCTTGAGCCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 440 bp | - | + | + |
| TUF | TUR | 56 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
