10397 Chgb Status: ES cell colonies screened / QC no positives
| Gene | Chgb |
| Chromosome | 2 |
| Deletion Start | 132,781,737 |
| Deletion End | 132,794,829 |
| Deletion Size | 13,093 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,781,849 | 132,781,870 | CGCCTCTCCTCTTCCCTGTATC |
| TUP | 132,781,872 | 132,781,892 | CTTGCCTTGCCTGGCCTGGCT |
| TUR | 132,781,901 | 132,781,921 | GCTGGACAGCTCTTGCTTCTC |
| TDF | 132,794,436 | 132,794,454 | TCCCATTCGGAGCCTTGAC |
| TDP | 132,794,457 | 132,794,482 | AATTCGATCATGTCAGGTGGTGCCTC |
| TDR | 132,794,503 | 132,794,524 | GGATCAGCAATCCATGTGACTC |
PCR Primers
| Primer | Sequence |
| SU | CGCATCCCTGCCCGTGCCTG |
| SD | AGAGCTAAGAACCAAAAAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 390 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 373 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
