10398 Apoa1bp Status: Cancelled
| Gene | Apoa1bp |
| Chromosome | 3 |
| Deletion Start | 88,058,487 |
| Deletion End | 88,056,574 |
| Deletion Size | 1,914 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,058,152 | 88,058,171 | ATGGAGCTGGCCGGGTTGAG |
| TUP | 88,058,129 | 88,058,150 | TGTGCCACGGCTATTGCCAAGG |
| TUR | 88,058,101 | 88,058,121 | TCACTGAGGTTTCTCGCTGTG |
| TDF | 88,056,754 | 88,056,777 | GGATGGGATGTAGAGAAGGGAAAC |
| TDP | 88,056,724 | 88,056,753 | CCTAGCGGAATCCAACCAGACTTACTCATC |
| TDR | 88,056,705 | 88,056,722 | TCTTGGGTGCCGTCAGTG |
PCR Primers
| Primer | Sequence |
| SU | TGCTTCAAGATGGTGGCCAGC |
| SD | TCTGAACACAAAGTAGATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 348 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 391 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
