10399 Ccl6 Status: Cancelled
| Gene | Ccl6 |
| Chromosome | 11 |
| Deletion Start | 83,592,938 |
| Deletion End | 83,588,721 |
| Deletion Size | 4,218 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,592,534 | 83,592,552 | GCAGGAGGCATGATGGTTG |
| TUP | 83,592,505 | 83,592,530 | TGTCTGACACCACCTCTACAAAGCCA |
| TUR | 83,592,446 | 83,592,468 | CGTGGTTAAATGGTCTTGGGTTG |
| TDF | 83,588,871 | 83,588,893 | CGGAGAGTTCAGAGGTGCCTAAG |
| TDP | 83,588,844 | 83,588,868 | CCCTGAAGCAAGGCCCAAGATCTGG |
| TDR | 83,588,807 | 83,588,824 | CAATGCCTGCCCTCCTTC |
PCR Primers
| Primer | Sequence |
| SU | CACCATGACCTTTGCCCTC |
| SD | GGACATATGTATGGCCTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 374 bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 352 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
