10401 Chac2 Status: Cancelled
| Gene | Chac2 |
| Chromosome | 11 |
| Deletion Start | 30,986,243 |
| Deletion End | 30,977,372 |
| Deletion Size | 8,891 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,986,215 | 30,986,236 | TTGGTTATGGGTCCCTCATCTG |
| TUP | 30,986,178 | 30,986,201 | CCCTACCAGGACAAGCTGGTTGGT |
| TUR | 30,986,152 | 30,986,172 | AAGCGCCGACTGTAGTTTGTG |
| TDF | 30,977,602 | 30,977,626 | GATTCCACAACAAAACCATTCAGTG |
| TDP | 30,977,583 | 30,977,601 | TTTTGCTCTACATTGGAAC |
| TDR | 30,977,550 | 30,977,571 | GGTGCAGGGCCAAGATAGTTAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCTCCAACTCGAGAGTC |
| SD | ATCACTTTTACAACGTGAACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 346 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 323 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
