| Gene | Wfdc5 |
| Chromosome | 2 |
| Deletion Start | 164,182,650 |
| Deletion End | 164,178,158 |
| Deletion Size | 4,493 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,181,993 | 164,182,013 | GAAGCTCGGGTTGGTTTGTTG |
| TUP | 164,181,963 | 164,181,988 | TGAGGGAGGCATAAGGGTTCTTCCAC |
| TUR | 164,181,916 | 164,181,937 | CTCAGAGGAGGATGGAGGGATG |
| TDF | 164,178,749 | 164,178,770 | AGCCTGCTTCCTCCAGTGTATG |
| TDP | 164,178,722 | 164,178,746 | TCGTGTCTTTGGTAAGTGCCCTCCC |
| TDR | 164,178,693 | 164,178,713 | TTGCTTGAGGTTTGGGTGTTG |
PCR Primers
| Primer | Sequence |
| SU | GTTCACATTCCCAGGCAGAG |
| SD | TATAACACTGGTTCTGAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10403A-A2 | VGB6 | C57BL/6NTac | |
| 10403A-D10 | VGB6 | C57BL/6NTac | |
| 10403A-B12 | VGB6 | C57BL/6NTac | |
