10404 Hist1h4i Status: Cancelled
| Gene | Hist1h4i |
| Chromosome | 13 |
| Deletion Start | 22,041,320 |
| Deletion End | 22,040,794 |
| Deletion Size | 527 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CGCCACCGCAAAGTGCTT |
| TUP | | | CGGGACAACATCCAGGGCATCACC |
| TUR | | | ATCACGTTCTCCAGGAACACC |
| TDF | | | TGTACGGGTTCGGCGGTT |
| TDP | | | AAGCTGCTCAGTTTCTT |
| TDR | | | AGCTCTTTTGATTTTGTGGTGGC |
PCR Primers
| Primer | Sequence |
| SU | CCACTTACATCCTATACAGTC |
| SD | TATGGACTGAACGAGGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
