10407 Hist1h2bh Status: Cancelled
| Gene | Hist1h2bh |
| Chromosome | 13 |
| Deletion Start | 23,543,349 |
| Deletion End | 23,542,972 |
| Deletion Size | 378 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGCTCCAAGAAGGCCC |
| TUP | | | TGACCAAGGCCCAGAAGAAGGACG |
| TUR | | | GTTCATGATGCCCATGGCT |
| TDF | | | TCGAGCGCATCGCGG |
| TDP | | | CGTCCCGCCTGGCGCATTACAAC |
| TDR | | | GAGCTGGTGTACTTGGTGACA |
PCR Primers
| Primer | Sequence |
| SU | ACGAGTCTTCATTTGAATAGAG |
| SD | TGACAGGGTTCTTAGAACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 356 bp | - | + | + |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
