10409 Ttc39a Status: Cancelled
| Gene | Ttc39a |
| Chromosome | 4 |
| Deletion Start | 109,407,158 |
| Deletion End | 109,444,205 |
| Deletion Size | 37,048 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,407,568 | 109,407,588 | TGAAGGGCTCTAAGTGTGCTC |
| TUP | 109,407,593 | 109,407,618 | CCAGGGTATTGGGTGTACCAGGAACC |
| TUR | 109,407,648 | 109,407,666 | GGCCAAGACAGCCATGTTG |
| TDF | 109,443,650 | 109,443,671 | TCCTAGCTGTACTCCCTTTGAC |
| TDP | 109,443,674 | 109,443,700 | AAGGTGCCCAGATATCAGTGAGGATGC |
| TDR | 109,443,704 | 109,443,725 | AGTTCCCAGAACACTGCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | CAGCTCCGGTCCATGTCTG |
| SD | TGAAGGCTTTGGCCACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
