10410 Tmem88 Status: Cancelled
| Gene | Tmem88 |
| Chromosome | 11 |
| Deletion Start | 69,398,222 |
| Deletion End | 69,397,593 |
| Deletion Size | 630 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,398,112 | 69,398,132 | ACGGCTCAGAATCTGCTGGTG |
| TUP | 69,398,080 | 69,398,105 | TTCAATCTCCTCTTGCTGGCACTGGT |
| TUR | 69,398,025 | 69,398,046 | GCAGAGAAAGCCGAAGCCTAAC |
| TDF | 69,397,894 | 69,397,913 | GTGGCCCATTCTGATGTCTC |
| TDP | 69,397,867 | 69,397,891 | ATCCGCACAGTTTCTGCGCTCTCAG |
| TDR | 69,397,811 | 69,397,831 | GTGACCAACAGGGCTGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGTGTTTTGATGATGGCT |
| SD | AAGGGAAAGAAGATGTGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 392 bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
