10412 Slc16a13 Status: Cancelled
| Gene | Slc16a13 |
| Chromosome | 11 |
| Deletion Start | 70,220,674 |
| Deletion End | 70,217,697 |
| Deletion Size | 2,978 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,220,620 | 70,220,641 | GGATGGATGGTGGTGCTCTCAG |
| TUP | 70,220,598 | 70,220,619 | CGTTCTTCCAGTCGGCGCTCGT |
| TUR | 70,220,550 | 70,220,572 | GCCACAAATTCCACGAAGAAGAC |
| TDF | 70,217,932 | 70,217,956 | TCACCCATGTATGTTTCTCTTTCTC |
| TDP | 70,217,911 | 70,217,931 | TTGGACCCAGGCTACCTCCGG |
| TDR | 70,217,886 | 70,217,905 | AAGCCGTGTAGTTGCCTGTC |
PCR Primers
| Primer | Sequence |
| SU | TGGTTTTATTTTCTGACCAC |
| SD | AGTCCTCAACAGAACATGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 394 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 252 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
