10414 Coro6 Status: Cancelled
| Gene | Coro6 |
| Chromosome | 11 |
| Deletion Start | 77,463,897 |
| Deletion End | 77,469,501 |
| Deletion Size | 5,605 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,464,779 | 77,464,799 | GGCCTGACAATAGCCTATGAC |
| TUP | 77,464,801 | 77,464,823 | CACGCTGGACAATGCAGACACCA |
| TUR | 77,464,848 | 77,464,865 | GGCTCGGGCTGAAATGTG |
| TDF | 77,469,069 | 77,469,091 | CAGGATGACCTATACCCAGACAC |
| TDP | 77,469,099 | 77,469,120 | CCTGAGCCTGCCCTGGAAGCTG |
| TDR | 77,469,151 | 77,469,171 | CCTTCAGCGAAATGAGCACAG |
PCR Primers
| Primer | Sequence |
| SU | TCCCGGCTAGCCTGTGTGTC |
| SD | TACGGAGCTCAGTCCCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 334 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | 282 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
