10415 Reep4 Status: ES cell colonies screened / QC no positives
| Gene | Reep4 |
| Chromosome | 14 |
| Deletion Start | 70,545,688 |
| Deletion End | 70,548,505 |
| Deletion Size | 2,818 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,546,132 | 70,546,154 | TCCCAATACCTGGCTCAAATGTC |
| TUP | 70,546,155 | 70,546,177 | CCTCACTGCATGCTGGTGGTCCT |
| TUR | 70,546,184 | 70,546,204 | CCGCTCCCACTACACGTAATC |
| TDF | 70,547,678 | 70,547,697 | GTGTATCGTGCAGGCAAAGG |
| TDP | 70,547,698 | 70,547,724 | AGCGCAGCTATGAAACCATGCTCAGTT |
| TDR | 70,547,738 | 70,547,756 | TGAGGCAGCGATGTTGAGG |
PCR Primers
| Primer | Sequence |
| SU | CCACCCGGACCCGAGACCAG |
| SD | GTTGGACTTTTGGTTTCGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 442 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 324 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
