10418 Tmem246 Status: Cancelled
| Gene | Tmem246 |
| Chromosome | 4 |
| Deletion Start | 49,587,163 |
| Deletion End | 49,585,894 |
| Deletion Size | 1,270 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,586,648 | 49,586,665 | TACGAGGGCACCGAAGAC |
| TUP | 49,586,613 | 49,586,642 | CGGTGATGACCCTTCCACTAACTCATTTGA |
| TUR | 49,586,560 | 49,586,579 | TGTAGGTTTGCAGCGATGAC |
| TDF | 49,586,006 | 49,586,026 | GAGCCCAACCTTGTGAAACAC |
| TDP | 49,585,982 | 49,586,003 | TGGGCTCTTCTCCAGCCTACGG |
| TDR | 49,585,939 | 49,585,960 | GCATTTCTTGGCACTCCTACAG |
PCR Primers
| Primer | Sequence |
| SU | TGCTGGGCTGCAGTGTTGG |
| SD | CACGAGCCAGAACTTGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 255 bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 353 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
