| Gene | Kncn |
| Chromosome | 4 |
| Deletion Start | 115,884,741 |
| Deletion End | 115,887,766 |
| Deletion Size | 3,044 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Taqman primers not yet available.
PCR Primers
| Primer | Sequence |
| SU | TGGCCAAAGTGGAAATGTGG |
| SD | AGGAACAAAGTTGAGGTGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 420 bp | - | + | + |
| NeoFwd | SD | 247 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
