10420 1810049H13Rik Status: Germline transmission achieved
| Gene | 1810049H13Rik |
| Chromosome | 11 |
| Deletion Start | 120,319,330 |
| Deletion End | 120,318,046 |
| Deletion Size | 1,285 |
| Genome Build | 37 |
|
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,318,837 | 120,318,858 | CTGGCTATCCGATCTGGACTTG |
| TUP | 120,318,808 | 120,318,835 | TCCTCCTGCGAACTTTGAGAGTGAATGC |
| TUR | 120,318,786 | 120,318,804 | TCGGCTCAATGCCTAACAC |
| TDF | 120,318,267 | 120,318,285 | GCTGCCACCTGAGCTTGAG |
| TDP | 120,318,241 | 120,318,264 | TCCCACCAACTGCTGCATGAGTGG
|
| TDR | 120,318,198 | 120,318,218 | CAGCAGAGCTTCGGCATAATC |
PCR Primers
| Primer | Sequence |
| SU | TGTCCCGAGCCTCTAAGAC |
| SD | GGAAAGCTGTAGCTGTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 307 bp | - | + | + |
| TUF | TUR | 52 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 328 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10420B-C5 | VGB6 | C57BL/6NTac | |
| 10420B-C8 | VGB6 | C57BL/6NTac | |
| 10420B-D3 | VGB6 | C57BL/6NTac | |
| 10420B-E12 | VGB6 | C57BL/6NTac | |
| 10420B-F3 | VGB6 | C57BL/6NTac | |
| 10420B-H9 | VGB6 | C57BL/6NTac | |
