10422 Sfn Status: ES cell colonies screened / QC no positives
| Gene | Sfn |
| Chromosome | 4 |
| Deletion Start | 133,601,766 |
| Deletion End | 133,600,951 |
| Deletion Size | 816 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,601,509 | 133,601,531 | AGGTGAAAGAGTACCGGGAGAAG |
| TUP | 133,601,480 | 133,601,502 | ACCGAGCTCAGAGGTGTGTGCGA |
| TUR | 133,601,460 | 133,601,476 | CCAGCAGGCCGAGTACG |
| TDF | 133,601,278 | 133,601,296 | CAAGAAGGAGATGCCGCCT |
| TDP | 133,601,254 | 133,601,274 | AACCCCATCCGCCTGGGCCTG |
| TDR | 133,601,143 | 133,601,161 | ACTGAGGGTGTGCAGGTCG |
PCR Primers
| Primer | Sequence |
| SU | CTGTCCTGGTGACTCACAG |
| SD | AACACCCACCAGACAGCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 355 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 154 bp | + | + | - |
| NeoFwd | SD | 228 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
