10423 Pramel1 Status: ES cell colonies screened / QC no positives
| Gene | Pramel1 |
| Chromosome | 4 |
| Deletion Start | 143,396,458 |
| Deletion End | 143,398,902 |
| Deletion Size | 2,445 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,397,171 | 143,397,191 | GAGGTGAAACAGCCAGTGAAG |
| TUP | 143,397,193 | 143,397,217 | TGCTGGTAGACTTGGTCCTCAAGGA |
| TUR | 143,397,228 | 143,397,248 | GAGGAAGGACTCTGTGGAATC |
| TDF | 143,398,707 | 143,398,731 | CAAGCAGAAATCCCAACAGTCAAAG |
| TDP | 143,398,734 | 143,398,762 | ACGCTTTATCCCAAGCTGTAGTCATCACA |
| TDR | 143,398,763 | 143,398,781 | GCCTCAAACTCCAGACCAC |
PCR Primers
| Primer | Sequence |
| SU | AAGATGTACTGTGGCAGTGG |
| SD | CCACTCCCATGACTTCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 366 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
