10426 1700093K21Rik Status: ES cell colonies screened / QC positives
| Gene | 1700093K21Rik |
| Chromosome | 11 |
| Deletion Start | 23,518,926 |
| Deletion End | 23,516,402 |
| Deletion Size | 2,525 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,518,101 | 23,518,120 | GAGCCACTGAAGACCCTCTG |
| TUP | 23,518,079 | 23,518,100 | TACAGGCGAAGGCTGCGTAGCT |
| TUR | 23,518,023 | 23,518,045 | CCCAGTCCCTAGACTTACTTGTC |
| TDF | 23,516,823 | 23,516,845 | CTCCCATTCCTCTGTAGCCTAAG |
| TDP | 23,516,794 | 23,516,821 | TGGGATACTAGGCCATATTGCTCTCAGC |
| TDR | 23,516,748 | 23,516,770 | TCCCTACATTGACACGGACTAAC |
PCR Primers
| Primer | Sequence |
| SU | CAGTGGGTCCAAGTTCAGTC |
| SD | CTTACGGTTATGCTAGACAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 329 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
